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Multiple Paternity and Clutch Size in the Guatemalan Beaded Lizard (Heloderma charlesbogerti) and the Black Beaded Lizard (H. alvarezi)


Mendelson III, Joseph R.

Zoo Atlanta

Georgia Institute of Technology

Atlanta, Georgia USA



Levine, Brenna A.

Department of Biology

Kean University

Union, New Jersey USA


Hill, Robert. L.

Zoo Atlanta

Atlanta, GA USA


Ramirez-Velazquez, Antonio

Zoologico Miguel Alvarez del Toro

Tuxtla Guittierez, Chiapas, Mexico


Otsuka, Nami

School of Biological Sciences

Georgia Institute of Technology

Atlanta, GA USA


Booth, Warren

Urban Evolutionary Entomology Lab

Department of Entomology

Virginia Polytechnic Institute and State University

Blacksburg, Virginia USA


A group of adult male and female Guatemalan Beaded Lizards (H. charlesbogerti) co-habited a large outdoor enclosure for five years during the species’ perceived breeding season. Through genomic parentage analysis, 50% of clutches comprising multiple offspring were found to result from multiple paternity, with up to three males siring offspring within single clutches. Both males and females were observed to produce offspring with multiple partners within a given year. As such, within this captive environment, where opportunities existed for mating with multiple partners, the genetic mating system was found to be highly polygamous, with multiple paternity common within clutches. These findings are novel for the family Helodermatidae, and the results have broader implications about how reproductive opportunities should be managed within captive conservation programs. A sample of 29 clutches (129 eggs, 41 hatchings) resulted in clutches numbering 1-11 eggs. A sample of 7 clutches (88 eggs, 31 hatchings of captive H. alvarezi, reported here for the first time, resulted in clutches number 1-13 eggs. Both of these ranges are in the same range as other Heloderma spp.

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